LOCUS EY192246 277 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa38g04.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY192246 VERSION EY192246.1 GI:159496663 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 277) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 931 was extended to value 931 (,) This trace has been recalled with phred original value before phred recall for SL was 153 original value before phred recall for SR was 476 PolyA tail found inside read...SR being truncated to 431 Potential full insert read...vec-to-vec length: 339 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..277 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggac acttctccaa ttcaaatcca aaaacaagaa attcgggatt ctttcttcct 61 ttaaatggtc cccacactct tgacgctatt tcccgcgctc gtgattgtca tgatgatggt 121 ggaggagccg gtgcaagcca gtcctaggcc ggactgcacc caaatgtgcc aagattctgc 181 ttgcagccag aaatgtaatc gcaaaaaacg ggatgaagca tttgcgcagc tatgcagcgg 241 aggcgtgtgc aagacggcga gcgcgagtac tggcaga //