LOCUS       EY192231                 196 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa36h10.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_563782.1| expressed protein [Arabidopsis
            thaliana] ref|NP_565691.1| expressed protein [Arabidopsis thaliana]
            sp|O80873|U123_ARATH Hypothetical protein At1g07170/At2g30000
            pir|T02485 hypothetical protein At2g30000 [imported] - Arabidopsis
            t, mRNA sequence.
ACCESSION   EY192231
VERSION     EY192231.1  GI:159496620
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 196)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            putative full insert read: vector-to-vector length is 292 (,)
            original QR value of 729 was extended to value 729 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 150
            original value before phred recall for SR was 442
            PolyA tail found inside read...SR being truncated to 347
            Potential full insert read...vec-to-vec length: 338
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..196
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggac tagtgacgcg tattattgca aggaatgcac aataatggag aaggacagga
       61 tggttgtcca aagattgtca atctaggaag tgccaaaacg gatttgttct acgagcggaa
      121 gaaatatggg tacaaaaaga cgtgaatttg aacccgatct ttcattattt attgaaaaat
      181 taaaagctgt atgata
//