LOCUS EY192215 294 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa36f02.g1 R.similis_EST_RSAA Radopholus similis cDNA 5' similar to emb|CAA84680.2| Hypothetical protein ZK1058.5 [Caenorhabditis elegans], mRNA sequence. ACCESSION EY192215 VERSION EY192215.1 GI:159496546 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 294) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 911 was extended to value 912 (,) This trace has been recalled with phred original value before phred recall for SL was 151 original value before phred recall for SR was 917 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..294 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggcaa gcggaattcg gataattttt tcaaagaccg gaactggact aaaaatgaac 61 tgcgggaatt gtgtagtgac ctggatctgg aggctacact catattaatt gaagctggat 121 gtggatgcgg aaacacgata ttccctctga aagctttttt cccgacctgg atcttctggg 181 gaatagactt ttcgaaaaat gcaatcgctt tacttaacga acgcgctaaa cgtgaaaaat 241 tggaagttgg cactggcgtg atggatctca ctgattgttc ctctctgccg ccgg //