LOCUS       EY191242                 309 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa12b10.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY191242
VERSION     EY191242.1  GI:159493612
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 840 was extended to value 841 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 152
            original value before phred recall for SR was 505
            PolyA tail found inside read...SR being truncated to 460
            Potential full insert read...vec-to-vec length: 447
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggta ttgcgggaaa aggagtcggc cattgtgatc aacaagattg acatggccga
       61 aaatcagaac gccttggaac gttcgattgc ggagatgttc ccggagaaga ggctcttatt
      121 catttcgggc agatatcgac aaaatcttga cccacttttg atctttctgc gtgaaaaaca
      181 ggaacaattc ttggctcaaa ggcaggcatt gctcgaaagg gaacgtgatg aaatgatgat
      241 ttaatacata ttttacttgt ttgtttttgt tctcaattcg acatttctaa tttcataaat
      301 ttacatgta
//