LOCUS       EY191232                 536 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa12a10.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_496426.1| Putative plasma membrane membrane
            protein, with at least 7 transmembrane domains, of eukaryotic
            origin [Caenorhabditis elegans] pir|T18769 hypothetical protein
            B0491.1 - Caenorhabditis elegans emb|CAA90090.1| Hypothetical
            protein, mRNA sequence.
ACCESSION   EY191232
VERSION     EY191232.1  GI:159493602
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 536)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 840 was extended to value 841 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 848
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..536
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggggc ggaaagcctc attccctcca gagttacaaa atctcctccg tcgcccggat
       61 gggcgtgtac gcttcattcc ttttcgagtt ttaacgatat gaatgactca aatactcttg
      121 cttctattcc tgccctagta agatggacaa atacaaaact acctgcactt gctttcttag
      181 ttagagtttt tttaattctt tacgcacata ttcacgacta cttatttaag gtcaatttta
      241 ctgatgtaga ttacgaagta ttttcggatg cagcttatct ggttttgcaa ggcaaatcac
      301 cgtatgttca tgctacgtac cggtacatgc catttgtttc ctggatgctg acaccaaatt
      361 attactttcc tgactttgga aaattcctct tttgtgctct cgatattttt gcagggtggt
      421 ttcatttccg cattgcgggg ttggactcga cctccaacga tgtgcgtatt cagaacagaa
      481 gccgttgggc tgtgatcatt tttctggctt tttaatcctt ttattgcaat aatctc
//