LOCUS       EY191224                 364 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa11h11.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_495755.1| Helicase HEL-1 (48.5 kD) (hel-1)
            [Caenorhabditis elegans] sp|Q18212|HE47_CAEEL Probable
            ATP-dependent RNA helicase p47 homolog pir|T19484 hypothetical
            protein C26D10.2 - Caenorhabditis elegans emb|CAA91120.1| C.
            elegans HEL-1 pro, mRNA sequence.
ACCESSION   EY191224
VERSION     EY191224.1  GI:159493594
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 364)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 831 was extended to value 832 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 147
            original value before phred recall for SR was 555
            PolyA tail found inside read...SR being truncated to 515
            Potential full insert read...vec-to-vec length: 436
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggcag acgcacaaac gctgaacagt gttcaagacc gattcgacgt gacaatctcc
       61 gaacttccag accaaatcga cgtgggcacc tacatcgagg cacgcggaaa caacgcagca
      121 gcagagtgat gagggagccg agtgcaacaa gacgagaccg aagggcgaga tgaagggcaa
      181 tttggattac attattggat cgctaaattt gcaccattct ctggatattt ttgttgatcg
      241 gattaatttg cgcatgtcca caccacctat ttcaagctat tataatcgcg agcaaaagct
      301 ccgactgttt ttacaatatt tgtcggtttt aaaattttca gttttgtgag taaaatgttt
      361 ctca
//