LOCUS       EY191221                 298 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa11h08.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_498282.1| ER lumen protein retaining receptor
            (24.7 kD) [Caenorhabditis elegans] sp|Q09473|ER21_CAEEL Putative ER
            lumen protein retaining receptor C28H8.4 pir|H88469 protein C28H8.4
            [imported] - Caenorhabditis elegans gb|AAA62292.1| Hypoth, mRNA
            sequence.
ACCESSION   EY191221
VERSION     EY191221.1  GI:159493591
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 298)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 850 was extended to value 851 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 147
            original value before phred recall for SR was 490
            PolyA tail found inside read...SR being truncated to 449
            Potential full insert read...vec-to-vec length: 370
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..298
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggggt tcatataggg cactttacat tctgaattgg gtgtatcgtt attacgcaga
       61 ggatttccta gacattatcg cagctgtttc tggaattgta cagacagttc tatatactga
      121 ctttttctat ctctatgtga gcaaagttgt gcaacaaagg cgcaacatcc aattgcctgc
      181 ttgactgatt gatgaaagaa gtaaaaagga agagaatgga aagccaaatg catcttacaa
      241 attacaatta caatgttgtt tgtttattta ttgaataatt tcgattattc aaaaaaga
//