LOCUS       EY191215                 337 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa11h02.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_495281.1| Uncharacterised protein family
            (UPF0139) [Caenorhabditis elegans] sp|Q09993|U139_CAEEL
            Hypothetical 12.8 kDa protein K10B2.4 in chromosome II pir|T16603
            hypothetical protein K10B2.4 - Caenorhabditis elegans
            gb|AAA68261.1| Hypothe, mRNA sequence.
ACCESSION   EY191215
VERSION     EY191215.1  GI:159493585
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 337)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 841 was extended to value 842 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 531
            PolyA tail found inside read...SR being truncated to 488
            Potential full insert read...vec-to-vec length: 409
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..337
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggaa cgtgcttggg atgatattct ctatgatggg actgatgatg aggctcaaat
       61 ggtgctcgtg ggttgcactg atttgctctt gggtcagctt tgccaacatg cgtgcctccg
      121 atgataccaa acaagtggtc agctcgttta tgctcagcat ctccgcagtg gttatgagct
      181 atctgcaaga tccaacaccg attgtgcctc cgtgggcctg aaggtctaat tcaagaattc
      241 aacaaggaca agagtgaaaa tgatcaaatt tgtagaggca aatattgtga tgctcctttt
      301 catttttttt tctcgcagtg agtaaataat gcttaca
//