LOCUS       EY191208                 444 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa11g06.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY191208
VERSION     EY191208.1  GI:159493578
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 444)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 864 was extended to value 864 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 151
            original value before phred recall for SR was 667
            PolyA tail found inside read...SR being truncated to 596
            Potential full insert read...vec-to-vec length: 516
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..444
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggggg cgcgcagtga atccaatcac caccattatg caaactttca ggacttttat
       61 ccgctcaatt catacaacag aatgttctga ataattgtaa taactactac ctagtcacct
      121 ggcgtagctc tctcctctgc ctcaaatagg caatgacatt ttacattgca tcgaaggcga
      181 atggtaccgc cagcaatgga actggaattg cggaagcgaa caaaatcggt ctctcgtcgc
      241 ccctcacggt cggctattgt gtgccgattg ggatttgcgt ggccgccctt ctctactact
      301 tgcggacgga cctccgcttt ttcagaaggt tcacactcag ataatcgaga aataatattt
      361 tggcaatgag cttagctgaa ccaatcccgg tctacttatt cctttttttg tgactttctc
      421 gcatgtttta aatgattatt cgca
//