LOCUS EY191203 282 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa11f12.g1 R.similis_EST_RSAA Radopholus similis cDNA 5' similar to ref|NP_504476.1| Type V secretory pathway protein (31.7 kD) [Caenorhabditis elegans] pir|T32529 hypothetical protein CD4.2 - Caenorhabditis elegans gb|AAB88340.1| Hypothetical protein CD4.2 [Caenorhabditis elegans] gb|AAP57298.1| cell death-relat, mRNA sequence. ACCESSION EY191203 VERSION EY191203.1 GI:159493688 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 282) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 851 was extended to value 852 (,) This trace has been recalled with phred original value before phred recall for SL was 149 original value before phred recall for SR was 473 PolyA tail found inside read...SR being truncated to 434 Potential full insert read...vec-to-vec length: 354 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..282 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggac aagcacatga gcaaggagat cgccacaaag ctgagcatcg agagcctgtc 61 gctgctgaag cacgccgatt tcaagcggaa tgagccctgt tcgttggcca ttgtttgcga 121 aatgatcgcc gcccatatgg acattccggc ggcggaattg gcggaagcca ccacagagaa 181 tgcgcaccga gtctacggat tgggcgggag gcagagagaa aattgaattt gctttcacat 241 tttagtgtta tttttttgtt gttgttaaat ttaaattcct ga //