LOCUS       EY191200                 412 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa11f09.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY191200
VERSION     EY191200.1  GI:159493685
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 412)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 847 was extended to value 848 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 606
            PolyA tail found inside read...SR being truncated to 563
            Potential full insert read...vec-to-vec length: 484
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..412
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggtgt tgttgtgtgt tttaccttca gcactttttg ctgatgtagt gggtattagc
       61 acagctgata cagtaagtgt aataatatgt aatgtggttg agtacatatg gaagtttggt
      121 ggaccgctta tgacagtggt aataataggg gctgctatgc tagcaatctt tggtaagatg
      181 cagtggtcag ctcttattgc attagctgtg tttacagctg tgttttttgg ggcgtcaagt
      241 atcttaacta tgtttattgg tactgttact ggtgttaatg tggggaattg taccatgagt
      301 cgtattacag actaaggtgg aagtttgatg gggcaacaac ttgtttgttg cttcctgctt
      361 atctcattga agagaggggg aagaggaaaa aagaataaga ggaaaagaat ga
//