LOCUS       EY191198                 449 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa11f07.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY191198
VERSION     EY191198.1  GI:159493683
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 449)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 853 was extended to value 853 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 643
            PolyA tail found inside read...SR being truncated to 599
            Potential full insert read...vec-to-vec length: 521
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggggg tgaaagcagt agcagttgtg ccaaagagga gggcaagagt gaagcgaaag
       61 agttactaaa accccacaaa aagtcggcca gatatcgtcc gcaccgccgt cacgttcgtt
      121 gcaaggatct gagtctgctt agcacagaac aaaagcgcgc cattttcggg acaacaatgg
      181 caacggatga agaagatccc aaaatggaag cggaagaggg cattacaaaa gaagagaaag
      241 aaccagaaaa agaggaagcg gagacggaca atttacgcac ttcgagcgtg acaatgagtg
      301 aatactttgc gcaaaaaatg gccgtacttg cgaagaagaa gaacacaaaa acgaagaata
      361 gggatgctgt ggaagagaat gaagatatcg tcggatgaat cacgcacgat ttttatgttg
      421 tcaattttat aaacttttaa tatgcaaca
//