LOCUS       EY191194                 362 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa11f03.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_504502.1| Aminomethyltransferase mitochondrial
            (43.9 kD) [Caenorhabditis elegans] pir|T25732 hypothetical protein
            F25B4.1 - Caenorhabditis elegans gb|AAB37080.1| Hypothetical
            protein F25B4.1 [Caenorhabditis elegans], mRNA sequence.
ACCESSION   EY191194
VERSION     EY191194.1  GI:159493679
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 362)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 862 was extended to value 863 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 150
            original value before phred recall for SR was 555
            PolyA tail found inside read...SR being truncated to 512
            Potential full insert read...vec-to-vec length: 434
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggga aaagaagtgc tttgtgaaga agcgtgttgg gctcatttct accaagggtg
       61 gacgtgcatc aagggcacac atgccaattg tggacccagt caataaggca gcagttggtt
      121 tcgtcacgtc aggctgtcca tcgcccacac tgggagcaaa cattgcaatg ggctatgtgg
      181 acaaggaatg cgcaaaagtg ggtacccagc tggtggtgga gactggtggg ggacgccaaa
      241 atgccgtgga agtgtgcaaa atgccgtttg tgcccagcaa atactttgtc gcgcagaaat
      301 aggaaaagaa caaaaaccaa cgtttttcat aaattattat tttgcaataa aagcctcatc
      361 ca
//