LOCUS EY191189 329 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa11e10.g1 R.similis_EST_RSAA Radopholus similis cDNA 5' similar to ref|XP_312670.1| ENSANGP00000020650 [Anopheles gambiae] gb|EAA07460.1| ENSANGP00000020650 [Anopheles gambiae str. PEST], mRNA sequence. ACCESSION EY191189 VERSION EY191189.1 GI:159493674 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 329) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 843 was extended to value 844 (,) This trace has been recalled with phred original value before phred recall for SL was 149 original value before phred recall for SR was 522 PolyA tail found inside read...SR being truncated to 480 Potential full insert read...vec-to-vec length: 401 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..329 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggat ttctcgggag ttgtaccaat tctgtgtgga cgccaaattg gtggatgccc 61 aacttgtggc caagtggaaa aagcaaggct tcgagaactt gtgctgtcta cgctgcatcc 121 agaccagaga caccaatttc ggcaccaact gcatttgccg tgttcccaag tcgaaactag 181 aagcgggtcg cgtgattgaa tgtgttcatt gcggatgtag aggatgttcc ggatgagcga 241 aaacatctga gatcattact gtaacatcac cgatcatgga tcttcttatt tatcatttat 301 ttgaacttgg aatgaatgaa tgtttttga //