LOCUS EY191187 349 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa11e08.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY191187 VERSION EY191187.1 GI:159493672 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 349) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 801 was extended to value 802 (,) This trace has been recalled with phred original value before phred recall for SL was 149 original value before phred recall for SR was 543 PolyA tail found inside read...SR being truncated to 499 Potential full insert read...vec-to-vec length: 421 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..349 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggcca ccgtcagagc aacagcagtg tcacccggtg cgggaacccc ccaaattgag 61 cggtttgtcc aaattgtttg ggacaaatct gtggccgttt ggcagacatt tgcaagcagc 121 gagagcgcct gctcaatcag ctgaacggca acgacggcgg cgagacggag ctgtacgacg 181 aggacctgct gatgacgccg catccgggag cgcagaaaaa gcaatgaaat ggaaacgaaa 241 cataaaaagg gaaccatttg aaatattcta ttgggaatgg gattcaatcc gaatgagagt 301 gctaatttgt attcgcacaa gttcacttaa tgaaagaatc tggaatcga //