LOCUS EY191170 290 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa11d03.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY191170 VERSION EY191170.1 GI:159493655 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 290) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center putative full insert read: vector-to-vector length is 412 (,) original QR value of 780 was extended to value 781 (,) This trace has been recalled with phred original value before phred recall for SL was 149 original value before phred recall for SR was 561 PolyA tail found inside read...SR being truncated to 440 Potential full insert read...vec-to-vec length: 362 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..290 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggctg tattacttcg tcctcaaggg ctggaaggat gacaccgaca agcgattgac 61 ccggattgag gagcatttcg accagcgatt gtcccggatt gaacagcttc tgacgccgac 121 caacacgaac atgccgattc cgaacaccaa cgcgccgatt ccgaacacca acgcgccgaa 181 cccgaaagag aatggccctt gattcctgtt caccgaaacg aacattaatt gattcttgac 241 tatggagtgt atggatagaa atggatatat taaatatatc atatatgaca //