LOCUS       EY191162                 275 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa11c06.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY191162
VERSION     EY191162.1  GI:159493647
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 275)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            putative full insert read: vector-to-vector length is 356 (,)
            original QR value of 754 was extended to value 754 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 505
            PolyA tail found inside read...SR being truncated to 426
            Potential full insert read...vec-to-vec length: 347
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..275
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggac gtccgcgaga aaatcgccaa cttcaagttc tgagatagat ggagtgagaa
       61 ccgcgaggac gaattatgtc agacggttgg agcgaacgaa attcgtgtaa atctctttaa
      121 ttaagtttca tcttgccata atcaaacact tcacatttta atttacacaa ttctgcttag
      181 tctataattc ctgtgctatt ttatgtgttc ttgtgctact tatgtgaaag cattcaacca
      241 aaaaacaaca acatgacggt aaaatttgct gttta
//