LOCUS       EY191147                 253 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa11b03.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY191147
VERSION     EY191147.1  GI:159493632
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 781 was extended to value 782 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 449
            PolyA tail found inside read...SR being truncated to 403
            Potential full insert read...vec-to-vec length: 325
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggggg taatggtgct ggcggggtcg gaggtgtgaa tgcggctgta gtagttcgac
       61 ctgggataat tgcacgcttg cgaaacatgt ttaatcttta accgacaaaa ttgacaaatt
      121 catggtctaa cttctttgct cagtgtcatc tacacattca atcttgacgg atactcaaat
      181 ttttacacca gtcatcagtt atttgactgt cttgaattat taactgtgat aatttgataa
      241 aataattttg ata
//