LOCUS EY191146 402 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa11b02.g1 R.similis_EST_RSAA Radopholus similis cDNA 5' similar to sp|Q9Y697|NFS1_HUMAN Cysteine desulfurase, mitochondrial precursor (HUSSY-08), mRNA sequence. ACCESSION EY191146 VERSION EY191146.1 GI:159493631 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 402) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 865 was extended to value 866 (,) This trace has been recalled with phred original value before phred recall for SL was 150 original value before phred recall for SR was 597 PolyA tail found inside read...SR being truncated to 552 Potential full insert read...vec-to-vec length: 474 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..402 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggtt gatggcgctc aaggatgtgg ccctgtcctc gggcagcgcc tgcacttcgg 61 ccagtctgga gccttcctac gtcctgagag ccattggggc ggatgaagac ctcgcgcact 121 cgtccatccg ctttggcatc ggccgcttca gcaccgaaaa ggaaatcgac ttcgtggtgg 181 ccaagtgcgt caaagaggct cttcgactgc gagagatgtc accgctctgg gaaatggtgc 241 aggaaggcat tgatctcaag acaatcaagt ggacccaaca ctgagcgacc gctaaaaatt 301 gtgccgttga cccgatgtgc taatcattcg atattttggc aaattttaaa tttcgatgga 361 attatgaatc aaattgtgta gagaaaataa tttatttgtt ca //