LOCUS       EY191145                 434 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa11b01.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_493578.1| Yeast MMS related MMS-2, Ubiquitin E2
            (conjugating enzyme) Variant UEV-1 (15.9 kD) (mms-2)
            [Caenorhabditis elegans] pir|T21984 hypothetical protein F39B2.2 -
            Caenorhabditis elegans emb|CAB07383.1| C. elegans UEV-1 protein
            (corresp, mRNA sequence.
ACCESSION   EY191145
VERSION     EY191145.1  GI:159493630
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 792 was extended to value 793 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 624
            PolyA tail found inside read...SR being truncated to 585
            Potential full insert read...vec-to-vec length: 506
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggtca ccacaaaata aacatgaatg gcgttaatca gcagaatgga attatcgaca
       61 aacgcgtggt gccgtcactt cgccaatgga acaattcata ctacataaag acggtgctcg
      121 aggacattcg aaggcacttg atgactgcca aggaaaatgc gaagctctct cacccagcag
      181 agggatcagt ctactgagtt gttttctcta tcaccggatc aggatggtca taaccgcatc
      241 gcacatcctt ccagtcatct acccacaacc accctttgcc cctaccccca aacgaccaat
      301 aataactctg actccccact ccccctgtca gccgatgtaa agatgttgcg ccctgatcaa
      361 cagatgattt gttccatttt cacattgttt tgtgttcttt actttgagac ttaatgattt
      421 gattttgacc aaga
//