LOCUS       EY191141                 249 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa11a09.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY191141
VERSION     EY191141.1  GI:159493626
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 249)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 861 was extended to value 861 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 148
            original value before phred recall for SR was 442
            PolyA tail found inside read...SR being truncated to 400
            Potential full insert read...vec-to-vec length: 321
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..249
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggcctgggga aaagcgcgcg cgtgtcctct cgcagtacgt gcccgaattc gcggcagagc
       61 cgaacttcaa cccgattgcc gctgcctgtt gagcgacaat cgacaccatt cgaattcaag
      121 tcctccgttg cgaaaaacat tccgcagata tataatgcga aaagacactc catgactcga
      181 tcagtgtaca ttcacaattt ctatcacgtt tctgattcca ttgtttgcga aagaataaac
      241 ttttattta
//