LOCUS EY191043 189 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa09h02.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY191043 VERSION EY191043.1 GI:159493485 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 189) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center putative full insert read: vector-to-vector length is 412 (,) original QR value of 887 was extended to value 888 (,) This trace has been recalled with phred original value before phred recall for SL was 149 original value before phred recall for SR was 561 PolyA tail found inside read...SR being truncated to 342 Potential full insert read...vec-to-vec length: 325 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..189 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggaa tacctgaaca aatctgtcgt tagaatttag aatctcccac agccgatttg 61 caacgtatca cacattgaga tttgagttgt tccaccgaat ctatttgatc caccactatt 121 tttgtgcgca ccccaaaaac tctaaatgct tccaatttat atgctttgac gaataaattt 181 ggttgttta //