LOCUS       EY191042                 554 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa09h01.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to gb|AAK85734.1| beta-G spectrin [Brugia malayi], mRNA
            sequence.
ACCESSION   EY191042
VERSION     EY191042.1  GI:159493484
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 554)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 869 was extended to value 870 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 876
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..554
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggac tcagagatgc gtacgcaggg gaaagagcaa tggagatcgt cagcaaagaa
       61 aatgaagtgc taaaggcttg gcgtcaactg cgagctgcat gtgaagcaag gactatgcga
      121 cttggcgaca cctcggacct gtttcgtttc atgaatatgg tgagggactt gtcggtgtgg
      181 atcgaagaag tgaagcggga aatgctatct cacgaacgac ctcgagatgt ttcgggggtg
      241 gaactgctga tgaacaatca tcaatcgttg aaagccgaaa ttgacactcg ggaagagaat
      301 ttccgcacat gtatcgctct gggaaggcaa ttgctcgaaa gagaccacta tgcaagtgcg
      361 gaagtggaga agaagttgat aaaactgacc accgatcgtg cggacatgat gcatcgttgg
      421 gaggaccgct gggagtactt gcgcctcatt ctggaagtgt accaatttgc acgtgatgct
      481 gcagtcgcag aagcatggct ggacgcacaa gaaccatact tacgatcgcg aaacttcggt
      541 cgtaatcttg aaga
//