LOCUS       EY191025                 440 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa09f08.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY191025
VERSION     EY191025.1  GI:159493467
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 440)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 868 was extended to value 869 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 631
            PolyA tail found inside read...SR being truncated to 590
            Potential full insert read...vec-to-vec length: 512
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..440
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggcaa acttcttcga ggggaatgga caaaccagtc acccgactca aaccttttgt
       61 tattgcacga tcagaagaac agaaacgagt attcggactt ggctatccga gcgttccaac
      121 agtcacggtg gacgaatggt tcaatcaaat gcagaaaaat ggtggttttg gagcgaaacc
      181 tttcacgatc cagagcaacc aaactaatgg tgaagagtca tcggatgaag acgaaaatgg
      241 aacaaaacaa gaagcaaaga gacaaagtga gattcgacga gatgaatgga aagagatgaa
      301 tccccgtggc tggggaaaca cctacaacaa gggatgatgg acgttgtacg gaaaaagtcg
      361 tgacataagg agtaacatct atcagtgtat tccacattgc gattgcattt gcaatcatcg
      421 ataaataatt ttattgacga
//