LOCUS       EY191017                 592 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa09e09.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_503542.2| Lipase class 3 precursor family member
            (38.5 kD) [Caenorhabditis elegans], mRNA sequence.
ACCESSION   EY191017
VERSION     EY191017.1  GI:159493459
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 592)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 871 was extended to value 871 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 839
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..592
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggatg ttctgacaga tgcacgacat gcgggttttc tagtcacatt taccggtcat
       61 tctcttggag gagcacttgc ctcgcttgca gccattcgga ctgtactgga cggactaaga
      121 tcaagtaata aggtcaaatt gtacacattt ggacaaccac gagttggtga tgcagcactc
      181 gcttttaaac atgacgaatt agtcccacat agtttccgag tcgtattccg gatggacatt
      241 gtcccgcata tgcccccttg tgccaaaaac gagaattatc atggtgtgcg tagtggagac
      301 gacagcaaac catgcaaccc cgttggacaa gggccctatc atcatggaac agaaatttgg
      361 tatccaaacg gaatgaatcc aggtgatcgg tattacgagt gcgtaggtac accgcgtgat
      421 gaggattttc actgcagtga catgctttct ttcgaggtct ccaaatatgc aacctacatc
      481 catgaccacc gtcactactt tgggcataag gtgccagcat ttggaaaact tggctgtgat
      541 ccgaataatc gtgcgttgga agagaatatg ccaaatgaac ctaagaagag ca
//