LOCUS EY191013 419 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa09e05.g1 R.similis_EST_RSAA Radopholus similis cDNA 5' similar to ref|NP_573252.1| CG6769-PA [Drosophila melanogaster] gb|AAF48775.1| CG6769-PA [Drosophila melanogaster] gb|AAL28670.1| LD10434p [Drosophila melanogaster], mRNA sequence. ACCESSION EY191013 VERSION EY191013.1 GI:159493455 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 419) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 960 was extended to value 960 (,) This trace has been recalled with phred original value before phred recall for SL was 152 original value before phred recall for SR was 618 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..419 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 gggcggggga cgacggctcc tacctcgtgc tcccgtccgg cgcgaggatc ggacatcgtt 61 ctctgatgca ctacttccac caaaagttgc gcccagtcca agaggaaggg gcaaatcatc 121 ccaaaatggc tggtcgttcg tcgctgactg gtaaactgaa gcaactgggc tggacggtca 181 caactggtgc acttgctgtt gctcatatgc gcgacatggc gtggatgaga aggaccgtgg 241 accgctacaa attccaacag agtctggcat ccaacaaact gttcttctcg cgcggacgcg 301 acgaccaaat gttaattcta tgaccgaagg atcataacga tcaaaattat tttgatttcc 361 gtgcactttg ttaccttttt cattgattga aattgatgaa taaaattgtt ttgttcgga //