LOCUS EY191006 72 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa09d09.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY191006 VERSION EY191006.1 GI:159493448 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 72) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 831 was extended to value 832 (,) putative full insert read: vector-to-vector length is 171 (,) This trace has been recalled with phred original value before phred recall for SL was 149 original value before phred recall for SR was 320 PolyA tail found inside read...SR being truncated to 222 Potential full insert read...vec-to-vec length: 144 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..72 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggcc acaatttgat cgagtattta agtatttatt taaaaacttc gttggaataa 61 aagagaaaaa ta //