LOCUS       EY191005                 444 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa09d08.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY191005
VERSION     EY191005.1  GI:159493447
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 444)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 808 was extended to value 809 (,)
            putative full insert read: vector-to-vector length is 554 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 703
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..444
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggat cactggatcg ttaacgagca atagaactac aaatggttta aggagcacca
       61 ttttctggac cgaatggttt ccatctgcta agctcctacc gccatgcacg cgctggacca
      121 tagctggccg atgggcccct tcatgaaggt gacttactcc ttgccggcca ccatccacat
      181 gacacgtgtg gactgctcta ggctggagga ggcgcgcaag aatgcgatca tccgggagct
      241 gcctgcgatg gagcaacact ctaagaaacg ggggattggc ctcctcctca agaacgactc
      301 tgatgatacg gctaatggct ccggtgctgg aggggcacag caccactgat gaaagacaca
      361 gcagcacctg catctaggtt ggcctggaaa ttgtgagagt gctcgcattt ttcttttgga
      421 ttctggatat gatcttttct ctcc
//