LOCUS       EY191000                 307 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa09d01.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY191000
VERSION     EY191000.1  GI:159493442
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 307)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            putative full insert read: vector-to-vector length is 352 (,)
            original QR value of 887 was extended to value 888 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 150
            original value before phred recall for SR was 502
            PolyA tail found inside read...SR being truncated to 460
            Potential full insert read...vec-to-vec length: 446
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..307
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggatt ttaggttata aataattaat gaagtataaa ctattaatgt tatatttttt
       61 agataaatta atttttctag taatttattt ttagataaat taatgttcca gaaatatcgg
      121 ctagacatta ttatttttaa ctaaaaactt tttaaatttt attttaattt atataaattt
      181 atattaatat aggtgaaatt ttaattataa ttatgttaat aattttataa aatttaaaat
      241 tttaattttt aacttaggtt agacactaat taatgaaatt taataatttt ctttagtaaa
      301 tttttga
//