LOCUS EY190998 282 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa09c11.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY190998 VERSION EY190998.1 GI:159493440 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 282) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 817 was extended to value 817 (,) This trace has been recalled with phred original value before phred recall for SL was 150 original value before phred recall for SR was 477 PolyA tail found inside read...SR being truncated to 434 Potential full insert read...vec-to-vec length: 422 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..282 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggaa tgaatgaagc aaaaagcact ctatgttcca tattttgact tgcaatttgc 61 aacggctgcc cacatttgtg tactattatt gcttggcttc actcgagacc aattctccaa 121 ttccacccgt acatttggat gtaccgaaca tttaaaagac gcacacactc aatctcaaag 181 gatcccatcc aaatacctat tattgatgat ggtcttttct gctgcacaaa gacaattttc 241 aaaaacatcg ttttgatgcg tttttgcaaa taaaaatgtg ta //