LOCUS       EY190993                 330 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa09c06.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_496985.1| Trp-asp repeats containing protein
            (90.1 kD) [Caenorhabditis elegans] emb|CAB76722.1| Hypothetical
            protein R06A4.9 [Caenorhabditis elegans], mRNA sequence.
ACCESSION   EY190993
VERSION     EY190993.1  GI:159493435
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 330)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 151
            original value before phred recall for SR was 526
            PolyA tail found inside read...SR being truncated to 482
            Potential full insert read...vec-to-vec length: 466
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..330
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggaat cacgggcgcg tccagtggcg aatttacact ttggaatgga acggcgttta
       61 acttcgagac gatccttcag gcccatgacg tggctattcg cgcgatggag tggtcccaca
      121 acgaccagtg gctggcgtcc gcggaccacg acggcttcgt caagtactgg cagccgaact
      181 tcaacaacgt gcacatgttc caagcgcaca aggacgaacc gattcggtcg ctaaggtgcg
      241 ccagagctgg ataaaaatgt tgtgtttttg tccgcgcaca atgtctcttc tcgagtcgtt
      301 ttggtctgca agtaaacttt aaccgataga
//