LOCUS       EY190992                 538 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa09c05.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY190992
VERSION     EY190992.1  GI:159493434
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 538)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 855 was extended to value 856 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 148
            original value before phred recall for SR was 731
            PolyA tail found inside read...SR being truncated to 688
            Potential full insert read...vec-to-vec length: 610
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..538
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggggt ggtagtagca aatattcaag tgagatcctt gaagactgaa gtggagaagg
       61 gttccacgtg aacagcagtt gaatgtgggt cagccgaacc taaggcaccg gctaacgccg
      121 tcacacacta cggggaagac tggtgctcgc acttgtccac ccggttgccg aaagggaagt
      181 gggttaacat tcccacgcca ggctggggag atggccgttc gcaagagcga ctacgcgtgg
      241 cgacacaaat gaactcgcag acgttgtgcg gagtccgggc cagagttctc ttttctggtt
      301 tagggcgcca gtccctggaa ttggctcagc ctgagatagg gacggcgcgc ccgtaaagca
      361 ccgcggttcc tgtggtgtcc caagcgctgc gcatgaccct tgaaaatgcg agggaagcgt
      421 tgtgaatctc ccgcctgctc gtaccgatat ccgcagcagg tctccaaggt gaacagcctc
      481 tagtcgatag aataatgtag gtaagggaag tcggcaaatt agattcgtaa cttcgaga
//