LOCUS       EY190985                 408 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa09b09.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY190985
VERSION     EY190985.1  GI:159493427
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 408)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 856 was extended to value 856 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 601
            PolyA tail found inside read...SR being truncated to 558
            Potential full insert read...vec-to-vec length: 480
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggag atagaattgt gttaaagtcg tcaatatccg aggcagaaaa gacggaagaa
       61 gcagtctcat tgatgagaat atcaaagaga cagaaggaag aagcggcgaa taaagaaacc
      121 gaagacgacg aatggtcgag tggagacgaa aaagagacga tgccgactgg actgtcgcag
      181 gacaagctgc atgaggagtt catctcaatg atggaggaga gattcatcga gggaagagat
      241 gcagaattct tcgattattc gaaggtggat gctggcattg gagagagtaa caaagagttc
      301 gacaaaatca aggagcaaga catggaagat gcatatttcg attatgaaga acccgaataa
      361 tgtaaatcac cccttccttc aaatatttgt taaattaaca gaattcga
//