LOCUS       EY190976                 488 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa09a09.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to emb|CAA74624.1| elongation factor-1d [Sphaerechinus
            granularis] emb|CAA74625.1| elongation factor-1d [Sphaerechinus
            granularis], mRNA sequence.
ACCESSION   EY190976
VERSION     EY190976.1  GI:159493418
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 488)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 879 was extended to value 880 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 681
            PolyA tail found inside read...SR being truncated to 639
            Potential full insert read...vec-to-vec length: 627
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..488
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggag gagcgcttga aggcctacgc ggagaagaag tccaagaagc ccggccccat
       61 cgccaagtcc aacgtgatct acgatgtcaa gccctgggac gacactgtgg acatcaagga
      121 gatcgagaag aatgtgcgct ctattcacac agaggggctt gtctggggca cttccaagat
      181 ccagccagtg gcattcggcg ttaagaagct tcagatttgc tgctgtgtgg aggacgaaaa
      241 ggtctcaact gactggctag aggagcagat cactggcttt gaggacctcg tccaatcggt
      301 cgatgtggtc gccttcaaca aagtctgatg agcgattgat gagcagcagg agcctattat
      361 tgactttcgc aggcttggtt tgggacccaa tatcacacat ttgttggcca ctcgcattgt
      421 tgacatttgg tttttgttgg tttatggcac aacatttaaa actttaaaag ttgataaaaa
      481 atatttta
//