LOCUS       EY190973                 622 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa09a06.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_508841.1| Actin ACT-4 (41.8 kD) (act-4)
            [Caenorhabditis elegans] sp|P10986|ACT4_CAEEL ACTIN 4 pir|S27135
            actin 4 - Caenorhabditis elegans emb|CAA34720.1| actin
            [Caenorhabditis elegans] gb|AAB04575.1| Actin protein 4, isoform a
            [Caenorhabd, mRNA sequence.
ACCESSION   EY190973
VERSION     EY190973.1  GI:159493415
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 622)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            putative full insert read: vector-to-vector length is 773 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 922
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..622
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggat caccgtcgga aacgagcgct tccgttgccc ggaggccctc ttccagccgt
       61 ccttcttggg catggagtct gccggcatcc acgagacgtc gtacaactcc atcatgaagt
      121 gcgacatcga catccgtaag gacctgtacg ccaacaccgt gctctctggc ggcaccacca
      181 tgtacccggg aattgccgac cgcatgcaga aggagatcac cgctctggcc ccgtcgacga
      241 tgaagatcaa gatcatcgct cctcctgagc gcaagtactc cgtctggatc ggaggctcca
      301 tcctggcctc gctgtccacc ttccagcaga tgtggatcag caagcaggag tacgacgagt
      361 cgggcccgtc catcgtgcac cgcaagtgct tctaagcagg gacagcggac agcgagacag
      421 acacagagga aggcagacag acagacaaca gaaaagtgct tcttttccac ccttccattt
      481 tttgcctgga aacttctttc ggagagaccc accatccacc acctttgttg ttcccatcca
      541 cacccaaccg ccacaaccca agatccatcc tcctcaccct cctgttgccg ctccctccgt
      601 ccctggccta gccgcgtaga gg
//