LOCUS EY190940 265 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa08e04.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY190940 VERSION EY190940.1 GI:159493534 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 265) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 861 was extended to value 862 (,) This trace has been recalled with phred original value before phred recall for SL was 152 original value before phred recall for SR was 461 PolyA tail found inside read...SR being truncated to 421 Potential full insert read...vec-to-vec length: 337 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..265 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggatt ctgcacatgg acggatttta cgaaggacaa ttgagcgttg acgaagtgaa 61 gatgcgtgtt gctgctgttg aagaatttgt gggccacgtg gcgaatcttc ccgcggaaat 121 tagtgacgaa aatcggcaga atctgatcga agccttgcgc aaatgaatat cgaatcatca 181 taaatcaaac gacaggaatc cactttcagc atgttatatc tcatctggct ttacattgtg 241 cacattttaa agaaaaattg ttgca //