LOCUS EY190934 250 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa08d09.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY190934 VERSION EY190934.1 GI:159493528 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 250) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 886 was extended to value 890 (,) This trace has been recalled with phred original value before phred recall for SL was 154 original value before phred recall for SR was 448 PolyA tail found inside read...SR being truncated to 404 Potential full insert read...vec-to-vec length: 389 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..250 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggat gatgctcgat aacgaggacc tgctccagac caaggtcaac gaggccgtca 61 ctgtgctcca gcaagcgaag ccgtctgctt gaagaaggac gcgagaagcg aagaaacaaa 121 aaaaagattg ggaagaaaag cgagatttcg ccaaaaaagg atgaaccaca ctattatttt 181 ttgtcaaaaa attgtatcct tttattttcg gccgtggcac tcatcaaatt gttgcataaa 241 tttttcggga //