LOCUS EY190933 438 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa08d08.g1 R.similis_EST_RSAA Radopholus similis cDNA 5' similar to ref|NP_506393.1| ZIP Zinc transporter (40.5 kD) [Caenorhabditis elegans] pir|T24290 hypothetical protein T01D3.5 - Caenorhabditis elegans emb|CAB03256.1| Hypothetical protein T01D3.5 [Caenorhabditis elegans], mRNA sequence. ACCESSION EY190933 VERSION EY190933.1 GI:159493527 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 438) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 917 was extended to value 917 (,) This trace has been recalled with phred original value before phred recall for SL was 152 original value before phred recall for SR was 635 PolyA tail found inside read...SR being truncated to 591 Potential full insert read...vec-to-vec length: 510 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..438 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggggc gcaaccgaac gtcacaacga tgcattctga cgatgccgaa ctgcactcat 61 cccagttgac gggtctaatt ctgctctttt ccggcggcac ttttctctac attgccacag 121 tccatgtgct gccagaactc atggaagcaa agtgcggcgg gatgggactg agtgcgagca 181 gcagcagaga gatgctgttg ggaggaggcg agaagagctc ttcgtccttc gccagatttt 241 ctctcttcga gctgaccctg ttctgcacct ctgcactaat tccggcgttc atatcctccc 301 ttgggcacgg ggcacacggc cacaaatgaa caaaagcatg atgcggtcgt tggttgtgat 361 ccttcactat ttttgatatt caataatggt ttctttgttt ttaccgacgc agctgtaaat 421 ataaataaat gcatgtta //