LOCUS       EY190924                 519 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa08b11.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to gb|AAM33245.1| mitotic phosphoprotein 36 [Xenopus
            laevis], mRNA sequence.
ACCESSION   EY190924
VERSION     EY190924.1  GI:159493518
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 519)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 818 was extended to value 818 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 148
            original value before phred recall for SR was 705
            PolyA tail found inside read...SR being truncated to 672
            Potential full insert read...vec-to-vec length: 638
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggggg aggctggtga accacagcta cttacgttca aatctcaaga ctaaggtgtt
       61 ggattttggg acacggttca atctggtgct gaccgccaaa cgggatatcg atgtcggcga
      121 ggagcttctc tatgactacg gagagcggag accataagtc gtggaaaggc acccctggct
      181 gctcaactcc tgatcacgtt attcagggat ctcttctcca ccgatcaatc ccaaattaat
      241 taattcctca tctttacaag ccaaatttgc gcattatcat cctgtgtgat gtattttggt
      301 gtaattatcg ttaccaaaca taattttgaa catgcgtcct cacttgcaat acctacctat
      361 ttaactttca actcattgtt tttgcctttc ctcgcccaaa ttctctcgat ccacttcatt
      421 tatgctttac tctgtcgcgt acaacttcaa atcgaaggat gttggtttgt catgtcattt
      481 tgattttaaa aacattgatg cataaaccga atcttgaga
//