LOCUS EY190906 154 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa07h04.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY190906 VERSION EY190906.1 GI:159493500 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 154) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 804 was extended to value 805 (,) This trace has been recalled with phred original value before phred recall for SL was 152 original value before phred recall for SR was 351 PolyA tail found inside read...SR being truncated to 312 Potential full insert read...vec-to-vec length: 291 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..154 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggggg caagattttg ggccaagaag tttagacaat accgatgttt ccgagaaaga 61 cccttttgat agaccactga acgccggttg tttttggacg gccataattt atttctaaga 121 ggattattaa acaaaataaa tttgcatact ttta //