LOCUS       EY190901                 500 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa07g10.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY190901
VERSION     EY190901.1  GI:159493495
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 500)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 885 was extended to value 885 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 148
            original value before phred recall for SR was 693
            PolyA tail found inside read...SR being truncated to 651
            Potential full insert read...vec-to-vec length: 572
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..500
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggcaa gctggaaggg acagtatctg agtgtcgacg gaatgatggt tcaaaacaaa
       61 gaaatcagac ttgcggttgc agccgaaatt gtcaaaattg caaaatctgt gaaggtgccc
      121 ggtactgatg atggctatcc aaagattggc tggactacga ggaagaatca aggttgccaa
      181 tgcgatgagt ttctcgaatc gttgaatgcc gagaacacgc gcaagacgac tggatggctt
      241 ctctttcgtt tgggaattga ccaatggtcg aatctttgtc ccaaatgaaa actgatgaaa
      301 atggagcaaa ttggagacac agtataaagc ccttttctct ttttcctcgt tctgctttat
      361 tttattgtga tgactgaatt tcttttttat cgtccattat gtttaggtat tattaattat
      421 tgtactttct ttttgtgaag tctcgtgtta atgcttgcta ttgcgataca aactcccacc
      481 gaataaatac aatagcggca
//