LOCUS       EY190900                 502 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa07g09.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY190900
VERSION     EY190900.1  GI:159493494
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 502)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 866 was extended to value 867 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 148
            original value before phred recall for SR was 691
            PolyA tail found inside read...SR being truncated to 652
            Potential full insert read...vec-to-vec length: 574
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..502
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggcc gcactgcaca aattcgacaa actgatcgtg agtcataccc cgcatcagtt
       61 ggtcgaatat cactgcgcat tgatccgctg gctcaacgcg aacaacaatg gccaaccgaa
      121 actgtgcatc ctcggctggt ttaccgagtt ggacaggaaa gttgctctgt tgctgctgga
      181 tgccataaaa aaggactttg ctggcgccac aaacaatcgc ccctttgtcg tgcagtttgc
      241 actgtacaca gactcattgg gcgagttcac catgagaaat gcgcacactg gggagcggct
      301 ccaatttcga aaccgccgga atgggttttg tcaattggtt cgatatccca ttgcgcagtg
      361 gcgcaaccac tatcaactgg atagggaatt tgaggacatc gacacgatga ccacaattct
      421 cggttaaatg agcagaataa tttgtttgtt ttcgattgaa gtggagtgag aatttcgggc
      481 aattgataaa cgagtagaag ta
//