LOCUS       EY190895                 497 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa07g04.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY190895
VERSION     EY190895.1  GI:159493489
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 497)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 847 was extended to value 851 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 150
            original value before phred recall for SR was 690
            PolyA tail found inside read...SR being truncated to 648
            Potential full insert read...vec-to-vec length: 569
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..497
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggacc agcgaatagt ccatcggctt ctaagccgaa gtccggtcgc ggtcgaggtc
       61 gccccaaggg ctccgtggcc ggagtaaaag aaggcggtgt ttccaagaaa aagggcggca
      121 ccagcggctt tgcaaaggga cgtggacgtc caaaaaaagc tgcaaatgcg gtccaagcgg
      181 cctctaacga gagtagtggt gaacagagcg ttggcaacaa tcgatcgcca aagacgtccc
      241 catccaagtc tccgtgaatt ttgtggatga agggacatgc atacctgaag gaatctcctc
      301 tccctggctc gacccacccc cctcactcat tccatctgac ttatgtaata taaactttta
      361 aatatgtaaa atgtagaact tattcccctc tctccacatc gacacaatag tttaaaaagg
      421 aatcgatacc ccgtaccccc gaacatcagt cttgatttca attcaacaat ggttttcttt
      481 aaataaatct tggatta
//