LOCUS       EY190889                 370 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa07f05.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY190889
VERSION     EY190889.1  GI:159493571
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 370)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 826 was extended to value 827 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 150
            original value before phred recall for SR was 563
            PolyA tail found inside read...SR being truncated to 520
            Potential full insert read...vec-to-vec length: 507
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..370
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggat gctgctcacg gaacgagggg aaagcagaca aagggcgcac gaacggctca
       61 aacaaatcgc cttgtggagc aatagtaatg ggaatggaaa caaacaggaa ctggagtcga
      121 cgtttgccga attgcctgag attggcgagg aattgcgcca aatgtgcgaa cagcccctcc
      181 agctgagtgg tcgtgcggcg gaacagactc gcgaattcat caacatcgaa ttgcgcccag
      241 ctattgccga agtgctcgca actgtgcagg acgctggaga gaggcgacaa ttggaaattt
      301 aggaccagaa attagactta aatttgttgc gatgaaacct tcttttgatt agtaataaaa
      361 cttggcccca
//