LOCUS EY190887 316 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa07f03.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY190887 VERSION EY190887.1 GI:159493569 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 316) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 875 was extended to value 883 (,) This trace has been recalled with phred original value before phred recall for SL was 149 original value before phred recall for SR was 510 PolyA tail found inside read...SR being truncated to 467 Potential full insert read...vec-to-vec length: 388 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..316 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggtgc acgagcaaat gctggctctg ttcggcacct accagcagtg ccagacgaag 61 gcgatatcgc agccgaaaaa gacgaaatag aggcacacag aggagaaaca atatcgagaa 121 tgcgtttaac gcagcgaacg gctttccaaa actctatctg gccgatttcc ctcccataac 181 ccctaagtcc aatcaccact tgaaagctgt acatttccgg agcccggagt ggggaattgc 241 ggaacgcgca gattgggggc atttgtttgc cacccgaggt atttgaccat tgttgtggga 301 ataaatttaa aaatca //