LOCUS EY190881 216 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa07e03.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY190881 VERSION EY190881.1 GI:159493249 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 216) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 743 was extended to value 744 (,) This trace has been recalled with phred original value before phred recall for SL was 149 original value before phred recall for SR was 405 PolyA tail found inside read...SR being truncated to 367 Potential full insert read...vec-to-vec length: 288 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..216 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggaa cgggaaataa gcgatgcctt caaggaagta gttggcgaag atggacttgc 61 ctcccgacaa aagcgactat tactgggatt gcactattaa agagaagtgg gacctagtgt 121 gccacccact acatttttcc attgatctct cactcacata gccggacatt tacctgggat 181 aattgaaagt gttcatggac aagaaggcta tgatta //