LOCUS       EY190880                 551 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa07e02.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_496536.1| DNA topoisomerase [Caenorhabditis
            elegans] sp|Q23670|TOP2_CAEEL PROBABLE DNA TOPOISOMERASE II
            pir|T23620 hypothetical protein K12D12.1 - Caenorhabditis elegans
            emb|CAA88867.1| Hypothetical protein K12D12.1 [Caenorhabditis
            elegans, mRNA sequence.
ACCESSION   EY190880
VERSION     EY190880.1  GI:159493248
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 551)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 919 was extended to value 919 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 160
            original value before phred recall for SR was 925
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..551
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggca caacttcaag ccggatccag tcaagaagtg gaaagacgag cagaaacgca
       61 aggaactgga agtgtgcgga gaggcgaatg tgtcggacga tgatgacgaa gaggtagagg
      121 ctgcgcctgc aacaggtgtt gaagctgcgc tggagaagcg gtgttcggat tacgactact
      181 tggttgggat ggctatctgg aagttgtcga tggaagataa agacaagctg ttggcagaaa
      241 gtgaatcgaa gaagaacgaa ctggagacat tgaagcgtaa acagtggagt gatttgtacg
      301 aggaggactt ggacagcttc ctcgcggcgt tggatactca ggaagaaaag gagcgcaagg
      361 acaatttgtt agagaaagct ggcaaggaga agggcggcgc caccaagaag aagggcgtag
      421 tgctcgatcc gaagttggcc aatgctccgc gccgtgagcc tcgcgagcct gatccaaact
      481 ttatccctga gaagaagccg aagaaaccgc aggagcccaa aaaggcaggc gcatcaaatg
      541 agaagaaaac a
//