LOCUS EY190876 365 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa07d09.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY190876 VERSION EY190876.1 GI:159493244 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 365) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 894 was extended to value 895 (,) This trace has been recalled with phred original value before phred recall for SL was 151 original value before phred recall for SR was 562 PolyA tail found inside read...SR being truncated to 516 Potential full insert read...vec-to-vec length: 508 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..365 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggcaa tcattttaca atacttacca aaaatggacg ctcaactcag ccagttcgtt 61 cttctccttg tttttatgct catgctgatc gccagcattc aattcgacgc tgtcgacgcc 121 caatgtgagg gcactctcac accggattgt ttgccgatct ggacttacgg gcgcagacgc 181 aagtgatgaa ttgacgaatg atggatgaag tgctgcgcag cacaaatcca caaatgttta 241 ggatgacaag aaactttttc aataaaaaaa caacaccttc tgctttctat ccaaaatgtg 301 tttcttgttt tcgtacctta catatgtacc tacctataat aaattttgat aaattgttaa 361 tttta //