LOCUS       EY190874                 390 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa07d07.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to sp|P41340|ACT3_LIMPO ACTIN 3 pir|S49480 actin 3 -
            Atlantic horseshoe crab emb|CAA86290.1| actin [Limulus polyphemus],
            mRNA sequence.
ACCESSION   EY190874
VERSION     EY190874.1  GI:159493242
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 390)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 892 was extended to value 892 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 584
            PolyA tail found inside read...SR being truncated to 540
            Potential full insert read...vec-to-vec length: 528
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggtg cgacatcgac atccgtaagg acctgtacgc caacaccgtg ctctctggcg
       61 gcaccaccat gtacccggga attgccgacc gcatgcagaa ggagatcacc gctctggccc
      121 cgtcgacgat gaagatcaag atcatcgctc ctcctgagcg caagtactcc gtctggatcg
      181 gaggctccat cctggcctcg ctgtccacct tccagcagat gtggatcagc aagcaggagt
      241 acgacgagtc gggcccgtcc atcgtgcacc gcaagtgctt ctaagcaggg acagcggaca
      301 gcgagacaga cacagaggaa ggcagacaga cagacaacag aaaagtgctt cttttccacc
      361 cttccatttt ttgcctggaa acttcttcca
//