LOCUS       EY190865                 571 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa07b11.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY190865
VERSION     EY190865.1  GI:159493233
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 571)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 843 was extended to value 844 (,)
            putative full insert read: vector-to-vector length is 640 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 148
            original value before phred recall for SR was 788
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..571
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggggc ggacaagttc gacatcaatc ttttgaagga gatatgcgag aaatacctca
       61 tggaggagag tgacaggtcg gctatattca ggttcaggat ggcggagaag tacggtcttg
      121 ttggactgaa gagcaagacc ttgcgtcgct tgaatccgca ggtattctgt tcgcatttca
      181 tcgaaaatgt tgaggagttt gcgaagctga gcgccccccc gaagaggacc tgggaccagc
      241 gcatgcactc gctctgcgcc aactatgcgt tctcgctcaa gagggccaga ttcgagaaag
      301 gcgagcggag caagcctaag agcagacacg acaagaatcg gagtcggtcc cgctccacct
      361 acagcagccg ctactcaact gtctcctcgg gctactccga gaacgaccaa aaggagcaca
      421 gccacagaag caaaagctcc tcgtccaccg agccggacat tgatgaacag ccctattatg
      481 gctcgagtcc tgattatctc gctgattttt gagcggattg aaagtcttta ttgtgtttaa
      541 acaatcacca tataccaata atgctgttga c
//